Rapid Diagnosis of HIV-1 virus by Near Infrared Spectroscopy: based on Partial least squares regression

Currently, the laboratory diagnostic tests available for HIV-1 viral infection are mainly based on serological testing which relies enzyme-linked immunosorbent assay (ELISA) blood HIV antigen detection and reverse transcription polymerase chain reaction (RT-PCR) specific RNA sequence identification. However, these methods expensive time-consuming, suffer from false positive and/or negative results. Thus, there is an urgent need developing a cost effective, rapid accurate method infection. In order to reduce barriers effective diagnosis, near-infrared spectroscopy (NIR) was used detect virus in human serum, specifically, three absorption peaks with dose-dependent at 1582nm, 1810nm 2363nm were found by multiple FBiPLSR test analysis HIV-nano HIV-EGFP, but not MLV. Therefore, we recommend use of as characteristic spectrum peak, early screening diagnosis serum HIV.